Toasted Jatropha curcas seed meal in Nile tilapia (Oreochromis niloticus) diet: Effect on growth, economic performance, haematology, serum biochemistry and liver histology

Wasiu Adeyemi Jimoh, Ahmed Ayodeji Ayeloja, Musa Idi-Ogede Abubakar, Yusuf Olatunji Yusuf, Mohammed Olayemi Shittu, Somrat Adeola Abdulsalami


The effect of dietary inclusion of differently timed dry heat-treated Jatropha curcas on the growth and economic performance of Nile tilapia, Oreochromis niloticus, was evaluated in a 56-day feeding trial. Five isonitrogenous and isolipidic dietary treatments (35% crude protein and 10% crude lipid) were made consisting of soybean meal (control) which was replaced by J. curcas seed meal toasted either 5 min or 10 min at 20 and 40% to make other four test diets. A total of 225 juveniles of O. niloticus were acclimatized for a week, weighed and allotted into five dietary treatments. Each treatment was replicated three times with fifteen fish per replicate. Fish were fed 5% body weight on two equal proportions per day for 56 days. Growth data were collected at two-week intervals. The results from the study indicated that there was significant difference (P<0.05) in the growth and economic performance parameters among the fish exposed to different dietary treatments. However, there was no significant variations (P>0.05) in the different growth and economic performance parameters of fish fed CTR and fish fed D520T (5 min toasted, 20%). There was significant reduction in haematological and biochemical parameters of the blood of O. niloticus fed the different dietary treatments containing J. curcas seed meal. Based on economic and physiologic performance, soybean meal in Nile tilapia diet could be replaced up to 40% by 5-minute toasted J. curcass seed meal.


Tilapia, Profit margin, Total cholesterol, Jatropha, Feed conversion ratio.

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(2010). Economic viability of replacing maize with whole cassava root meal in the diet of hybrid cat-fish. Agricultural Journal, 5(1): 1-5.

Adebayo O.O., Daramola O. A. (2013). Economic analysis of catfish (Clarias gariepinus) production in Ibadan metropolis. Journal of Agriculture and Food Sciences, 1(7): 128-134.

Adeparusi E.O., Balogun A.M. (1999). Roasted pigeon pea (Cajanus cajan) bean as a dietary protein source for Clarias gariepinus (Burchell, 1822). Applied Tropical Agriculture, 4(31-36).

Akinwande A., Dada A., Sogbesan O., Umar I. (2016). Haematological response of Heterobranchus longifilis fed varying dietary protein levels. African Journal of General Agriculture, 2(1): 17-21.

Alatise S., Adedokun M., Adelodun O., Ajiboye G. (2014). Effects of boiled jatropha kernel meal as a substitue for soyabeans meal in diet of african mud catfish (Clarias gariepinus) Fingerlings. Journal of Fisheries and Aquatic Science, 9: 446-454.

AOAC. (2010). Official methods of analysis, Vol. 18th Edition, Revision 3. Association of official analytical chemists, Washington DC. Gaithersburg, Maryland: AOAC International.

Arellano-Martinez M., Racotta, I., Ceballos-Vazquez, B. P., Elorduy-Garay, J. (2004). Biochemical composition, reproductive activity and food availability of the lion's paw scallop Nodipecten subnodosus in the Laguna Ojo de Liebre, Baja California Sur, Mexico. Journal of Shellfish Research, 23(1): 15-24.

Azaza M., Wassim K., Mensi F., Abdelmouleh A., Brini B., Kraϊem M. (2009). Evaluation of faba beans (Vicia faba L. var. minuta) as a replacement for soybean meal in practical diets of juvenile Nile tilapia Oreochromis niloticus. Aquaculture, 287(1-2): 174-179.

Barcellos L.J.G., Kreutz L., Rodrigues L., Fioreze I., Quevedo R., Cericato L., Conrad J., Soso A., Fagundes M., Lacerda L.D.A. (2003). Haematological and biochemical characteristics of male jundiá (Rhamdia quelen Quoy & Gaimard Pimelodidae): changes after acute stress. Aquaculture Research, 34(15): 1465-1469.

Boateng V.F., Alhassan E.H., Saahene Y., Nensom E., Abarike E.D. (2013). Profitability analysis of all-male tilapia farming in Sekyere South and Bosomtwe Districts of Ashanti Region. Agriculture and Biology Journal of North America, 4(5): 568-575.

Coz-Rakovac R., Strunjak-Perovic I., Hacmanjek M., Lipej Z., Sostaric B. (2005). Blood chemistry and histological properties of wild and cultured sea bass (Dicentrarchus labrax) in the North Adriatic Sea. Veterinary Research Communications, 29(8): 677-687.

Davies S., Gouveia A. (2008). Enhancing the nutritional value of pea seed meals (Pisum sativum) by thermal treatment or specific isogenic selection with comparison to soybean meal for African catfish, Clarias gariepinus. Aquaculture, 283(1-4): 116-122.

Fagbenro O., Adedire C., Owoseeni E., Ayotunde E. (1993). Studies on the biology and aquaculture potential of feral catfish Heterobranchus bidorsalis (Geoffroy St. Hilaire 1809)(Clariidae). Tropical zoology, 6(1): 67-79.

Fagbenro O., Akande T., Fapounda O. (2003). Use of Roselle (Hibiscus Sabdariffa) seed meal as a soybean meal replacer in practical diets for fingerlings of African Catfish (Clarias gariepinus). Paper presented at the Proceeding of the third international conference on African fish and Fisheries, Cotonou, Benin.

Fagbenro O., Smith M., Amoo A. (2001). Acha (Digitari exilis stapf) meal compared with maize and sorghum mea as a dietary carbohydrate source for Nile Tilapia (Oreochromis niloticus). Journal of Aquaculture, 52: 1-3.

Fagbenro O.A., Adeparusi E.O., Jimoh W.A. (2013). Haematological profile of blood of African catfish (Clarias gariepinus, Burchell, 1822) fed sunflower and sesame meal based diets. Journal of Fisheries and Aquatic Science, 8(1): 80-86.

Fakunle J., Alatise S., Effiong B., Tiamiyu K. (2013). Effects of replacing soyabeans meal with graded levels of boiled jatropha kernel meal in diets of Clarias gariepinus fingerlings. Bulletin of Environment, Pharmacology anf Life Sciences, 2(9): 112-117.

Fasakin, E., Balogun, A. (1996). Replacement of groundnut cake with processes soybean meal in diets for the clariid catfish Clarias gariepinus fingerlings. Applied Tropical Agriculture, 1: 5-11.

Faturoti E. (1989). Effects of supplementary feeding and organic manuring on the production of African catfish: Clarias gariepinus (Burchell 1822). West African Fish, 187-195.

Francesco F., Satheeshkumar P., Senthil Kumar D., Caterina F., Giuseppe P. (2012). Comparative study of hematological and blood chemistry of Indian and Italian Grey Mullet (Mugil cephalus Linneaus 1758). HOAJ Biology, 1-5.

Hoseini S.M., Vatnikov Y.A., Kulikov E.V., Petrov A.K., Hoseinifar S.H., Van Doan H. (2019). Effects of dietary arginine supplementation on ureagenesis and amino acid metabolism in common carp (Cyprinus carpio) exposed to ambient ammonia. Aquaculture, 511: 734209.

Hoseinifar S.H., Sun Y.-Z., Zhou Z., Van Doan H., Davies S.J., Harikrishnan R. (2020). Boosting immune function and disease bio-control through environment-friendly and sustainable approaches in finfish aquaculture: herbal therapy scenarios. Reviews in Fisheries Science and Aquaculture, 1-19.

Hoseinifar S.H., Zou H.K., Van Doan H., Kolangi Miandare H., Hoseini S.M. (2018). Evaluation of some intestinal cytokines genes expression and serum innate immune parameters in common carp (Cyprinus carpio) fed dietary loquat (Eriobotrya japonica) leaf extract. Aquaculture Research, 49(1): 120-127.

Hosseini S.A., Hoseini S.M. (2012). Effect of acute crowding stress on subsequent osmotic challenge and recovery in juvenile common carp Cyprinus carpio (Linnaeus). Comparative Clinical Pathology, 21(5): 583-588.

Jimoh W., Aroyehun H. (2011). Evaluation of cooked and mechanically defatted sesame (Sesamum indicum) seed meal as a replacer for soybean meal in the diet of African catfish (Clarias gariepinus). Turkish Journal of Fisheries and Aquatic Sciences, 11(2): 185-190.

Jimoh W., Ayeloja A., Ajasin F., Okemakin F., Abdusalami S., Adekunle O. (2015a). Some haematological and biochemical profile of blood of Nile tilapia (Oreochromis niloticus) fed on diets containing watermelon (Citrullus lanatus) seedmeal. Bayero Journal of Pure and Applied Sciences, 8(1): 109-114.

Jimoh W., Popoola M., Ayeloja A., Adedeji F. (2012). Impact of feeding diets containing Luffa cylindrica seedmeal on the variable cost of production of African catfish, Clarias gariepinus (Burchell 1822). In: 27th Annual Conference and Biennial General Meeting of the Fisheries Society of Nigeria (FISON), 25-30 Nov 2012 ,Bayelsa, Nigeria. pp: 24-27.

Jimoh W., Shittu M., Owolade E., Ojutalayo S., Arilesere, J., Bernard A., Ayeloja A. (2015b). Production economics of using watermelon seedmeal in the diet of Nile tilapia (Oreochromis niloticus) fingerlings. Applied Tropical Agriculture, 20(1): 96-101.

Jimoh W.A. (2004). Replacement value of jackbean meal for soybean meal in Nile Tilapia (Oreochromis niloticus) diets. (M.Sc Dissertation), Federal University of Technology Akure.

Jimoh W.A., Ayeloja A.A., Rifhat A.O., Adeleke A.B., Shodamola M.O. (2013). Incidence of cost analysis of producing African Catfish, Clarias gariepinus (Burchell 1822) using diets containing water melon seedmeal (Citrullus lanatus). Paper presented at the Proceeding of the 47th Annual Conference of Agricultural Society of Nigeria (ASN), Federal College of Animal Health and Production Technology, Moor Plantation, Ibadan.

Jimoh W.A., Shittu M. O., Ayeloja A.A., Okemakin F.Y., Abdulsalami S.A., Adekunle O.F., Banjoko O.J. (2015c). Histological changes in the liver of Nile tilapia (Oreochromis niloticus) fed diets containing watermelon (Citrullus lanatus) at varying replacement levels. Journal of Science and Technology, 6(1): 85-92.

Jimoh W.A., Shodamola M.O., Idowu S.D., Shittu M.O., Olawepo K.D. (2014). Incidence of cost analysis of producing Clarias gariepinus with diets containing Chrysophyllum albidum seedmeal as maize replacer. Paper presented at the Conference Proceedings of the 4th Biennial Conference of Forest and Forest Products Society held at Federal University of Agriculture, Abeokuta, Nigeria between April 23rd and 25th, 2014.

Kavadias S., Castritsi‐Catharios J., Dessypris A. (2003). Annual cycles of growth rate, feeding rate, food conversion, plasma glucose and plasma lipids in a population of European sea bass (Dicentrarchus labrax L.) farmed in floating marine cages. Journal of Applied Ichthyology, 19(1): 29-34.

Kumar V., Makkar H., Becker K. (2008). Detoxification of Jatropha curcas seed meal and its utilization as a protein source in fish diet. Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology, 151(1): S13-S14.

Kumar V., Makkar H., Becker K. (2011). Detoxified Jatropha curcas kernel meal as a dietary protein source: growth performance, nutrient utilization and digestive enzymes in common carp (Cyprinus carpio L.) fingerlings. Aquaculture Nutrition, 17(3): 313-326.

Luquet P. (2017). Tilapia, Oreochromis spp.. Handbook of Nutrient Requirements of Finfish, CRC Press. pp: 169-180.

Makkar H.P., Kumar V., Becker K. (2012). Use of detoxified jatropha kernel meal and protein isolate in diets of farm animals. In: Biofuel co-products as Livestock Feed – Opportunities and challenges, FAO, Rom. pp: 351-378.

Martínez-Llorens S., Baeza-Ariño R., Nogales-Mérida S., Jover-Cerdá M., Tomás-Vidal A. (2012). Carob seed germ meal as a partial substitute in gilthead sea bream (Sparus aurata) diets: amino acid retention, digestibility, gut and liver histology. Aquaculture, 338: 124-133.

Olagunju F., Adesiyan I., Ezekiel A. (2007). Economic viability of cat fish production in Oyo State, Nigeria. Journal of Human Ecology, 21(2): 121-124.

Paray B.A., Hoseini S.M., Hoseinifar S.H., Van Doan H. (2020). Effects of dietary oak (Quercus castaneifolia) leaf extract on growth, antioxidant, and immune characteristics and responses to crowding stress in common carp (Cyprinus carpio). Aquaculture, 735276.

Singh D., Singh A. (2002). Biochemical alteration in freshwater fish Channa punctatus due to latices of Euphorbia royleana and Jatropha gossypifolia. Environmental Toxicology and Pharmacology, 12(3): 129-136.

Storebakken T. (2000). Soy products as fat and protein sources in fish feeds for intensive aquaculture. Soy in Animal Nutrition, 127-170.

Workagegn K.B., Ababbo E.D., Tossa B.T. (2013). The effect of dietary inclusion of Jatropha curcas kernel meal on growth performance, feed utilization efficiency and survival rate of juvenile Nile tilapia. Journal of Aquaculture Research and Development, 4(5): 1.

Yousefi M., Hoseini S.M., Vatnikov Y.A., Kulikov E.V., Drukovsky S.G. (2019). Rosemary leaf powder improved growth performance, immune and antioxidant parameters, and crowding stress responses in common carp (Cyprinus carpio) fingerlings. Aquaculture, 505: 473-480.

Yousefi M., Shabunin S.V., Vatnikov Y.A., Kulikov E.V., Adineh H., Hamidi M.K., Hoseini S.M. (2020). Effects of lavender (Lavandula angustifolia) extract inclusion in diet on growth performance, innate immunity, immune-related gene expression, and stress response of common carp, Cyprinus carpio. Aquaculture, 515: 734588.

Yue Y.-R., Zhou Q.-C. (2008). Effect of replacing soybean meal with cottonseed meal on growth, feed utilization, and hematological indexes for juvenile hybrid tilapia, Oreochromis niloticus × O. aureus. Aquaculture, 284(1-4): 185-189.


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