A comparative study of the histoarchitecture of endocrine pancreas in Labeo bata (Hamilton, 1822), Sperata aor (Hamilton, 1822) and Chitala chitala (Hamilton, 1822)

Saroj Kumar Ghosh, Padmanabha Chakrabarti, Shrabani Barun


The disposition and cellular organization of the endocrine pancreas were studied in three species of freshwater teleosts viz., Labeo bata (Hamilton, 1822), Sperata aor (Hamilton, 1822) and Chitala chitala (Hamilton, 1822) using histological techniques. In L. bata, the endocrine pancreas tissues were mainly distributed in the adipose tissue among the intestinal coils and adjacent to extrahepatic bile duct, while in S. aor and C. chitala, the endocrine pancreas predominantly attached with wall of the stomach along with exocrine pancreatic part. Histological analysis demonstrated that the endocrine components of all the three species were enclosed in a thin capsule provided with different cells, interspersed with blood sinuses. The cytoarchitectural analysis showed that in L. bata, β cells were usually arranged in groups while α cells were often interspersed with blood vessels. In S. aor and C. chitala, the rounded or oval α cells were usually arranged either in groups or scattered to the islets periphery and β cells which were densely granulated and typically stained with Aldehyde fuchsin (AF), Romies azan (RA) and Mallory’s triple (MT) were observed in the central areas of the islets and intercalated with blood vessels. The δ like cells were founded at a low frequency and intermingled with β cells and exhibited moderate cytoplasmic granules in L. bata, S. aor and C. chitala. Despite being the subject of extreme controversy, the nature and function of different islet cells were discussed.


Histoarchitecture, Endocrine pancreas, Labeo bata, Sperata aor, Chitala chitala.

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Boquist L., Patent G. (1971). The pancreatic islet of the teleost Scorpaera scropha an ultrastructural study with particular regard to fibrillar granules. Zeitschrift für Zellforsch, 115: 416-425.

Brinn J.E., Epple A. (1972). Structure and ultrastructure of the specialized islet organ of the American eel, Anguilla rostrata. The Anatomical record, 172: 277.

Falkmer S. (1961). Experimental diabetes research in fish: on the morphology and physiology of the endocrine pancreatic tissue of the marine teleost Cottus scorpius with special reference to the role of glutathione in the mechanism of alloxan diabetes using a modified nitroprusside method. Acta Endocrinologica, 59: 1-122.

Falkmer S., Ostberg Y. (1977). Comparative morphology of pancreatic islets in animals. In: B.W. Volk, E.R. Arquilla (Eds.). The diabetic pancreas. Plenum Press, New York. pp: 15-58.

Halami N.S. (1952). Differentiation of the two types of basophils in the adenohypophysis of the rat and the mouse. Stain Technology, 27: 61.

Iaglov V.V. (1978). Morphology of the endocrine portion of the teleost pancreas. Arkhiv Anatomii, Gistologii I Embriologii, 74: 111-115.

Khanna S.S., Mehrotra B.K. (1968). Histology of the islets of Langerhans in normal and alloxan-treated freshwater Indian teleost, Clarias batrachus. Zoologische Beiträge, 14: 489-497.

Khanna S.S., Singh T. (1971) Histology of the principal islets of a freshwater teleost, Channa punctatus. Acta Anatomica, 78: 99-106.

Khanna S.S., Gill T.S. (1973). Histology of the principal islets in some freshwater teleosts. Anatomischer Anzeiger, 133: 367-376.

Kobayashi K., Takahashi Y. (1970). Light and electron microscope observation on the islets of Langerhans in Carassius carassius longsdorfii. Archivum Histologicum Japonicum, 31: 433-454.

Kobayashi K., Takahashi Y. (1974). Fine structure of Langerhans islet cells in a marine teleost Conger japonicas. General and Comparative Endocrinology, 23: 1-18.

Kudo S., Takahashi Y. (1973). New cell types of the pancreatic islets in the crusian carp, Carassius carassius. Zeitschrift für Zellforsch, 146: 425-438.

Lazarow A. (1963). Functional characterization and metabolic pathways of the pancreatic islet tissue. Recent Progress in Hormone research, 19: 489-546.

Mallory F.B. (1936). The aniline blue collagen stain. Stain Technology, 11: 101.

Mokhtar D.M. (2015). Histological, histochemical and ultrastructural characterization of the pancreas of the grass carp (Ctenopharyngodon idella). European Journal of Anatomy, 19: 145-153.

Plisetskaya E.M., Pollock H.G., Rouse J.B., Hamilton J.W., Kimmel J.R., Gorbman A. (1985). Characterization of coho salmon (Oncorhynchus kisutch) insulin. Regulatory Peptides, 11: 105-116.

Plisetskaya E.M., Pollock H.G., Rouse J.B., Hamilton J.W., Kimmel J.R., Gorbman A. (1986). Isolation and structures of coho salmon (Oncorhynchus kisutch) glucagon and glucagon-like peptide. Regulatory Peptides, 14: 57-67.

Sayrafi R., Najafi G., Rahmati-holasoo H., Hooshyari A., Akbari R., Shokrpoor S., Ghadam M. (2011). Histological study of hepatopancreas in Hi Fin pangasius (Pangasius sanitwongsei). African Journal of Biotechnology, 10: 3463-3466.

Shyamasundari K., Raja Kumari V.J.V., Hanumantha Rao K. (2006) Observation on the pancreas of the marine lizard fish Saurida tumbil (Bloch). Journal of Fish Biology, 21: 449-454.

Sorokin A.V., Petrenko O.I., Kavsan V.M., Kozlov Y.I., Debabov V.G., Zlochevskij M.L. (1982). Nucleotide sequence analysis of the cloned salmon preproinsulin cDNA. Gene, 20: 367-376.

Wang Y.Q., Plisetskaya E, Baskin D.G., Gorbman A. (1986). Immunocytochemical study of the pancreatic islets of the Pacific salmon, Oncorhynchuys kisutch. Zoological Science, 3: 123-129.

Youson J.H., Al-Mahrouki A.A. (1999). Ontogenic and phylogenic development of the endocrine pancreas (islet organ) in fishes. General and Comparative Endocrinology, 116: 303-335.


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